Vol 4-1 Review Article

Light-Field Microscopy: A Review

Oliver Bimber* and David C. Schedl

Faculty of Engineering and Natural Sciences, Johannes Kepler University Linz, 4040, Austria

Light-field microcopy (LFM) supports single-shot volumetric recording and instant 3D excitation. It is an emerging technology for fast wide-field 3D imaging of neuronal activity, and has the potential to enable scanless 3D photostimulation for applications in optogenetics. This article introduces the basics of light-field technology, reviews the LFM principles and current implementations, summarizes first applications for optical imaging and photostimulation of neural activity, and outlines alternatives that go beyond LFM.

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