Sema7A Signaling is Essential for Activity-Dependent Synapse Formation in the Mouse Olfactory Bulb

Nobuko Inoue1, 2, Hitoshi Sakano2*

1Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, 2-11-16 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan

2Department of Brain Function, University of Fukui School of Medicine, 23-3 Shimo-aizuki, Matsuoka, Fukui 910-1193, Japan

Odorant molecules are detected by olfactory sensory neurons (OSNs) in the olfactory epithelium. Odor information is then transmitted to the olfactory cortex through synapses with mitral/tufted (M/T) cells. We recently analyzed a pair of signaling molecules, Semaphorin (Sema) 7A expressed in OSN axons and its receptor Plexin (Plxn) C1 localized to M/T-cell dendrites. In the knockout mice for Sema7A or PlxnC1, initiation of synapse formation is perturbed. Rescue and reconstitution experiments demonstrated that interactions of Sema7A and PlxnC1 are essential to induce the post-synaptic assembly. Pharmacological blocking of NMDA receptors indicated that synaptic transmission induces primary-dendrite selection after the synapses are formed. We conclude that Sema7A/PlxnC1 signaling is key for initiating synapse formation followed by dendrite selection in M/T cells. Since other Sema molecules are known to regulate targeting of OSN axons without involving neuronal activities, Sema7A is a unique example of Sema family proteins that regulates synapse formation and dendrite selection in an activity-dependent manner. Possible roles of Sema7A/PlxnC1 signaling will be discussed in the context of olfactory circuit formation in neonates.

The mammalian olfactory system can detect a variety of odors using approximately 1,000 odorant receptor (OR) species1. It is well-established that each olfactory sensory neuron (OSN) expresses one functional OR gene in a mono-allelic manner2-5. Furthermore, OSN axons expressing the same type of OR converge to a specific target site, glomerulus, in the olfactory bulb (OB)6-8. As a given odorant interacts with multiple OR species and a given OR responds to multiple odorants2, binding signals of odor molecules detected in the olfactory epithelium (OE) are converted to a unique set of activated glomeruli, a odor map, in the OB7, 9, 10. This conversion enables the brain to detect and discriminate a large repertoire of odorants11.

Since the discovery of OR genes in 1991 by Buck and Axel1, it has well been studied how OSN axons target to the OB for glomerular map formation12-25. During development, a coarse olfactory map is generated by a combination of dorsal-ventral (D-V) projection and anterior-posterior (A-P) projection. D-V projection is regulated by positional information of OSNs in the OE12-19, while A-P projection is instructed by OR molecules using cAMP as a second messenger20, 21. Different sets of Neuropilin (Nrp), Semaphorin (Sema), and Plexin (Plxn) molecules are known to regulate these processes19, 21-25: A-P projection is regulated by Nrp1 and Sema3A26, and D-V projection is by Nrp2 and Sema3F19. In addition to these targeting processes that occur in an activity-independent manner, olfactory circuits are further refined by OSN activity. Multiple sets of axon sorting molecules whose expression is regulated by OR-derived OSN activity, are responsible for glomerular segregation27.

Although primary projection autonomously takes place26 even in the absence of the target OB28, proper connections are needed with mitral/tufted (M/T) cells in the OB to make the olfactory circuit functional. For synapse formation, pairing of OSN axons with nearby M/T cells takes place and then post-synaptic events are initiated in M/T-cell dendrites. Once the synapse is formed, primary dendrites are selected in a competitive manner. Synaptic activity is assumed to have a critical role in stabilizing the dendrite structure29. In contrast to the targeting of OSN axons, little was known about the events that lead to the formation of synapses with M/T-cell dendrites and a number of important questions were to be answered. For example, what kind of molecules mediate triggering of synapse formation and how are the synaptic structures selected and maintained in an activity-dependent manner? In an effort to address these questions, we recently studied signaling molecules, Sema7A and its receptor PlxnC1, essential for synapse formation and dendrite selection in M/T cells30. In this mini-review, we will briefly summarize our recent work on Sema7A/PlxnC1 signaling in the mouse olfactory system.

Various molecules, e.g., Neurexins / Neuroligins, ephrin / Eph receptors, and Teneurins, are known to be involved in synaptic connections and circuit formation in various systems31-36. In the mammalian olfactory system, however, it is poorly understood how the OSN axons are connected with M/T-cell dendrites for synapse formation. How are these two types of cells matched, and what kind of signaling molecules are essential for triggering the post-synaptic events within glomeruli? We searched for a receptor and ligand pair expressed in OSN axons and M/T-cell dendrites in neonates. Almost forty axon guidance, cell adhesion, and signaling molecules were analyzed for their expression in the neonate OE and OB. Among them, we found a promising pair, Sema7A and its receptor PlxnC1 expressed in OSN axons and M/T-cell dendrites, respectively30. Sema7A is a membrane-bound, glycosyl-phosphatidylinositol anchor protein37, functioning in the immune and central nervous systems38. Our immuno-electron microscopic study revealed that Sema7A is indeed localized to the pre-synaptic axon terminal of OSNs. It was found that expression levels of Sema7A vary among different glomeruli. This indicates that the Sema7A gene is regulated by the OR-derived neuronal activity as found for glomerular segregation molecules27. Activity dependency of Sema7A expression was confirmed by analyzing of mutant mice lacking CNG-A2, a component of cyclic nucleotide-gated (CNG) channels39. We also analyzed the glomerulus expressing the DRY-motif mutant-OR that was unable to couple with G proteins21. It was found that expression levels of Sema7A were significantly reduced in the glomeruli expressing the G-protein coupling mutant of OR30.

What is in vivo function of Sema7A within glomeruli? We generated and analyzed the Sema7A conditional-knockout (cKO) specific to OSNs expressing rI7 receptor30. Since the KOs of other Sema molecules severely affect targeting of OSN axons19, 21-25, we examined whether glomerular map was normally formed in the Sema7A cKO. Close examination of rI7-expressig OSNs did not reveal any sign of impairment in targeting of OSNs to the OB. However, when M/T cells were analyzed, synapse formation and dendrite selection were perturbed. In the Sema7A total knockout (KO)40, the synapse number stayed low and post-synaptic density (PSD) was rarely found in the M/T-cell dendrites. We then examined pre- and post-synaptic markers in the synapses of Sema7A cKO and total KO. Immunostaining revealed that both markers were markedly reduced in these KOs. We also analyzed dendrite morphology of M/T cells in the Sema7A cKO by injecting Lucifer Yellow into the rI7 glomeruli. Unlike in the WT, dendrites remained branched and multiple dendrites were often observed for single M/T cells in the cKO. This observation indicates that Sema7A expressed by OSNs plays important roles not only in inducing post-synaptic events but also in selecting primary dendrites of M/T cells.

We next studied how the Sema7A signal is processed in M/T cells. PlxnC1 is known to serve as a receptor for Sema7A in other systems40, 41. In Purkinje cells, Sema7A was reported to facilitate synapse elimination of mossy fibers via PlxnC142. In our study, immunohistochemistry of OB sections detected accumulation of PlxnC1 in the glomerular layer, indicating that PlxnC1 produced by M/T cells is localized to their tuft structures of dendrites within glomeruli. It should be noted that PlxnC1 is detected in the M/T-cell dendrites only during the early neonatal period. Since both Sema7A and PlxnC1 are expressed in the membrane-bound form, it is likely that they directly interact with each other and function as signaling molecules to initiate synapse formation. In order to study the in vivo function of PlxnC1, we generated the M/T-cell-specific cKO for PlxnC1. In the OB, M/T-cell migration and dendrite extension occurred normally in the PlxnC1 cKO, and targeting of OSN axons was also normal. However, initial assembly of synaptic structures was impaired and dendrite selection was delayed as seen in the Sema7A KOs whose impairments remained into adulthood. These results demonstrate that PlxnC1 is also needed to initiate the synapse formation and dendrite selection in M/T cells.

How does the Sema7A-PlxnC1 interaction induce the post-synaptic events? We studied PSD formation triggered by Sema7A using the heterologous system in vitro. The secrete-type of Sema7A and myc-tagged PlxnC1 genes were introduced into HEK293 cells in culture, where the secreted Sema7A interacts with PlxnC1 expressed on the HEK-cell surface. To analyze the aggregation of PSD, cells had been transfected with the FLAG-tagged gene for SAP90 that serves as a scaffold molecule in clustering the PSD32, 43, 44. PSD formation is the earliest hallmark of post-synaptic events in M/T-cell dendrites32, 45, 46. Co-localization of SAP90 with PlxnC1 was found by immunostaining of FLAG and myc tags, when the full-length PlxnC1 was expressed with the secrete type of Sema7A. In contrast, with the mutant PlxnC1 deleting the cytosolic domain, co-localization of SAP90 was not seen. We then examined whether the Sema7A-PlxnC1 interaction is indeed essential for triggering PSD formation. Based on the three-dimentional analysis of Sema7A47, we introduced the amino acid substitution, Y→S at residue 213, into the interaction site of Sema7A with PlxnC1. In our experiment, co-localization of SAP90 with PlxnC1 was not induced with the mutant Sema7A. This observation further supports the idea that PlxnC1 serves as a receptor for Sema7A in its interaction between OSN axons and M/T-cell dendrites. Physical interactions between Sema7A and PlxnC1 were also confirmed by using fusion proteins with alkaline phosphatase. These results demonstrate that Sema7A/PlxnC1 signaling recruits SAP90, and that the Sema7A-PlxnC1 interaction is essential for triggering post-synaptic events in M/T cells (Fig. 1A).

As mentioned earlier, it is not only the initiation of synapse formation, but also primary dendrite selection that is perturbed in the Sema7A KOs and PlxnC1 cKO. This is probably because the trans-synaptic activity from OSN axons is needed for dendrite selection in M/T cells after the establishment of functional synapses. To examine this, we used an NMDA-R inhibitor, MK801, to pharmacologically inhibit Ca2+ entry into M/T cells48. This treatment blocks synaptic activity after synapses have been formed. In our experiment, transcripts of the cpg15 gene, a marker for synaptic activity49, were measured in M/T cells after MK801 treatment. We found that MK801 significantly reduced cpg15 expression and suppressed dendrite selection. These results indicate that synaptic transmission, but not Sema7A/PlxnC1 signaling directly, promotes synaptic competition, causing dendrite selection and branch pruning in M/T cells (Fig. 1B).


Figure 1: Schematic diagrams of synapse formation and dendrite selection regulated by Sema7A/PlxnC1 signaling.

(A) Sema7A/PlxnC1 signaling is essential for triggering post-synaptic events in M/T cells. OR-derived neuronal activity of OSNs induces Sema7A expression in OSN axons. Sema7A interacts with its receptor PlxnC1 localized in M/T-cell dendrites and recruits PSD molecules.

(B) Synaptic transmission promotes synaptic competition, causing dendrite selection in M/T cells. After establishing synapses between OSN axons and M/T-cell dendrites, trans-synaptic activities from OSNs induce dendrite selection and branch pruning in M/T cells.

Since the discovery of OR genes1, many groups have studied OR-instructed axonal projection of OSNs, and major processes of glomerular map formation have been elucidated12-27. In contrast, olfactory circuit formation mediated by the secondary neurons, M/T cells, has long been elusive. What kind of molecules are involved in synapse formation with OSNs, and how is targeting of M/T cells regulated to various areas in the OC? Our recent studies revealed that interactions between Sema7A expressed in OSN axons and PlxnC1 in M/T-cell dendrites are essential for triggering synapse formation within glomeruli. Since other Sema molecules in the olfactory system are known to regulate targeting of OSN axons independently from neuronal activity19, 21-25, Sema7A described in our study is a unique example of Sema family proteins, which triggers synapse formation in an activity-dependent manner.

We found that the interaction of Sema7A with PlxnC1 induces postsynaptic events within glomeruli by recruiting SAP90 to assemble the PSD. Then, how is it that Sema7A/PlxnC1 signaling recruits PSD molecules? One possibility is a conformational change in PlxnC147 caused by binding of Sema7A to PlxnC1. This may induce the tyrosine phosphorylation that allows the assembly of PSD molecules. It is also possible that Sema7A-PlxnC1 interactions activate signal transduction pathways. Recent studies demonstrate that transmembrane molecules induce PSD formation by their own kinase activity, and not by recruiting PSD molecules via PSD domains50. A most likely scenario is that Sema7A/PlxnC1 signaling activates the PAK-Rac1/Cdc42 pathway30, 51, 52 which in turn regulates vesicle trafficking, cytoskeletal dynamics, and gene expression necessary for the post-synaptogenesis in M/T-cell dendrites. Then, how does pre-synaptic maturation occur in primary axons? As GPI-anchored Sema7A has no cytosolic domain in OSNs, it is unlikely that Sema7A signaling directly induces maturation of pre-synaptic structures. We assume that PSD-maturation induces pre-synaptic formation in a retrograde manner by using other membrane proteins or permeable factors. These processes in both pre- and post-synaptic events need to be clarified in the future.

It is interesting that Sema7A expression is enhanced by odorous ligands during the neonatal period. When pups are exposed to vanillin, a ligand to MOR29A53, Sema7A expression is increased and synapse formation is enhanced in the MOR29A glomeruli. Environmental odors, e.g., maternal smell, may help pups to establish specific neural circuits by up-regulating Sema7A expression for their survival. Activity-dependent expression of Sema7A may be beneficial for the adaptation of pups to the postnatal environment during olfactory circuit formation. Another intriguing possibility is olfactory imprinting during the critical period. It is possible that enhanced odor inputs mediated by Sema7A signaling may help establish the imprinted odor memory at early neonatal stages. These possibilities need to be studied in the future.

In the mammalian olfactory system, primary neurons are constantly regenerated replacing old OSN axons with new ones. In contrast, secondary neurons, M/T cells, are not replaced and glomerular structures are kept stably throughout the lifetime54, 55. It is interesting to study how the synapses are regenerated in adults using pre-existing glomerular structures. In the neuromuscular junction, the post-synaptic structure is maintained without the pre-synapse56, and interneurons help the post-synaptic structures to survive57. Synapse formation of regenerating OSN axons may be regulated in a different manner than that in the neonatal animal. How do the regenerating OSN axons find their target glomeruli in adults? Do they follow the pre-existing axons of OSNs expressing the same type of OR? These studies on olfactory map regeneration will give us a new insight into our understanding of the olfactory disfunction Parosmia caused by physical damage that destroys the wiring of OSN axons.

In our study, we described two types of neuronal activities: one is the OR-derived activity that regulates Sema7A expression in OSN axons (Figure 1A) and the other is trans-synaptic activity for dendrite selection in M/T cells (Figure 1B). In the mouse olfactory system, proper olfactory circuits may be established by selecting primary dendrites of M/T cells in an activity-dependent manner. It is interesting to further investigate whether M/T cells are naïve with respect to circuit formation that drive innate olfactory behaviors. It is important to determine how much of such processes are genetically programmed and how much can be modulated by environmental inputs. These interesting questions are expected to be answered in the future. The mouse olfactory system will continue serving as an excellent model system for the study of decision making and memory formation in the mammalian brain.

We would like to thank H. Nishizumi, H. Naritsuka, and H. Kiyonari for their collaboration in this study. This work was supported by a Specially Promoted Research Grant and grants in aid from MEXT of Japan. N. I. was a pre-doctoral fellow of the Japan Society for the Promotion of Science and supported by Japan Foundation for Applied Enzymology.

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Article Info

Article Notes

  • Published on: October 12, 2018


  • Olfactory bulb

  • Olfactory epithelium


Dr. Hitoshi Sakano
Department of Brain Function, University of Fukui School of Medicine, 23-3 Shimo-aizuki, Matsuoka, Fukui 910-1193, Japan